3D Cell Culture Models
Fig. 19.1 Representative image of a spheroid showing the key features. MCF7 cells seeded at 5000 cells per well in a 96-well ULA plate exhibited a tight spheroid after 96…
ONCOLOGY
Fluorescence Imaging of Tumors in Human Patient-Derived Orthotopic Xenograft (PDOX) Mouse Models
Fig. 15.1 (a) Human pancreatic-cancer-patient tumor with RFP and GFP stromal cells. Image was obtained with the Olympus FV1000. Green arrows indicate GFP stromal cells from GFP mouse. Red arrows…
Patient-Derived Xenograft Models of Prostate Cancer
Fig. 8.1 Three unique PDX models of early-stage, well-differentiated, androgen-responsive prostate cancer, respectively, all expressing AR and PSA [22, 37] The significant expansion of the number of PDXs for prostate…
Afterward: The Future of Patient-Derived Models of Cancer
© Springer International Publishing AG 2017Robert M. Hoffman (ed.)Patient-Derived Mouse Models of Cancer Molecular and Translational Medicinehttps://doi.org/10.1007/978-3-319-57424-0_21 21. Afterward: The Future of Patient-Derived Models of Cancer Robert M. Hoffman1, 2 (1) AntiCancer, Inc., 7917 Ostrow Street,…
Why Patient-Derived Mouse Models Need to Be Orthotopic
Fig. 20.1 Drug efficacy testing in the PDOX model of HER-2-expressing cervical carcinoma. PDOX nude mice were treated with saline, carboplatin, trastuzumab + lapatinib, trastuzumab + lapatinib + entinostat, or…
Use of Patient-Derived Orthotopic Xenografts (PDOX) to Evaluate Transformative Cancer Therapeutics
Fig. 13.1 Patient sarcomas and melanomas were obtained at surgery, established in nude mice and subsequently implanted orthotopically in nude mice and treated with drugs indicated [26, 27, 30–34, 57]….
Cervical Cancer PDOX Models
Fig. 10.1 Drug-efficacy testing in the PDOX model. PDOX nude mice were treated with saline, carboplatin, trastuzumab + lapatinib, trastuzumab + lapatinib + entinostat, or entinostat alone. (a) Bar graphs…
The Revival of Patient-Derived Xenograft Mouse Models of Cancer: Way Back to the Future
© Springer International Publishing AG 2017Robert M. Hoffman (ed.)Patient-Derived Mouse Models of Cancer Molecular and Translational Medicinehttps://doi.org/10.1007/978-3-319-57424-0_2 2. The Revival of Patient-Derived Xenograft Mouse Models of Cancer: Way Back to the Future Robert M. Hoffman1, 2 …
In Memoriam: Jørgen Rygaard (1934–2016), Father of Patient-Derived Mouse Models of Cancer and Modern Cancer Research
The athymic “nude” mouse is possibly the most important tool in cancer research. The nude mouse has enabled the studies of human cancer in the laboratory in vivo. Nude mice…
Techniques for Surgical Orthotopic Implantation of Human Tumors to Immunodeficient Mice
© Springer International Publishing AG 2017Robert M. Hoffman (ed.)Patient-Derived Mouse Models of Cancer Molecular and Translational Medicinehttps://doi.org/10.1007/978-3-319-57424-0_6 6. Techniques for Surgical Orthotopic Implantation of Human Tumors to Immunodeficient Mice Robert M. Hoffman1, 2 (1) AntiCancer, Inc.,…
