Identification and Biology of CML Stem Cells
Fig. 1.1 Clonal expansion of CML hematopoiesis. A reciprocal translocation between chromosomes 9 and 22 occurs at a hematopoietic stem cell (HSC) stage. This pre-leukemic stem cell expresses BCR-ABL at…
Monitoring of Anticoagulant Therapy
Anticoagulant Mode of action Plasma half-life Monitoring test(s) Therapeutic range Reversal Notes Warfarin (vitamin K antagonists) Interference with post-translational γ-carboxylation of vitamin K-dependent clotting factors 20–60 h INR 2.0–3.0, moderate…
Validation of Hemostasis Assays, Analyzers, and Reagents
Fig. 4.1 Example of a validation checklist 4.3 Validation Studies In the hemostasis laboratory, validation studies encompass the establishment of analytical performance expectations; calibration and calibration verification; determination of quality…
Hemostasis Screening Assays
Fig. 5.1 Platelet satellitism and platelet clumping in a blood smear from a patient with EDTA-pseudothrombocytopenia (Courtesy Sherrie L. Perkins, MD, University of Utah Medical Center [Wright’s stain, oil immersion])…
Testing for Inherited and Acquired Thrombotic Disorders
Fig. 9.1 Regulation of thrombin activity by vascular endothelium. The effects of major antithrombotic properties of the blood vessel wall are shown. Regulation of thrombin activity is important because thrombin…
Testing for Inherited Bleeding Disorders
Disorders associated with: Thrombocytopenia Platelet dysfunction (qualitative platelet disorders) Coagulation disorders Autosomal recessive Autosomal recessive X-linked Bernard-Soulier syndrome Bernard-Soulier syndrome Factor VIII deficiency (hemophilia A) Congenital amegakaryocytic thrombocytopenia Glanzmann’s thrombasthenia…
Coagulation Testing and Transfusion Medicine
Fibrinogen deficient curve % Activity Fibrinogen, mg/dL PT (s) (normal 10.7–15.0 s) PTT (s) (normal 25–40 s) 100 288 11 28 80 230.4 11.4 29.8 70 201.6 11.6 29.8 40…
Pharmacogenetic Testing for Anticoagulant and Antiplatelet Therapies
CYP2C9 VKORC1 *1/*1 *1/*2 *1/*3 *2/*2 *2/*3 *3/*3 GG Typical Typical Moderate ↓ Moderate ↓ Moderate ↓ Significant ↓ AG Typical Moderate ↓ Moderate ↓ Moderate ↓ Significant ↓ Significant…
Collection of Coagulation Specimens
Hematocrit, % Blood volume (excluding citrate), mL 4.5 2.7 1.8 0.9 56 0.41 0.24 0.16 0.08 57 0.40 0.24 0.16 0.08 58 0.39 0.23 0.16 0.08 59 0.38 0.23 0.15…
Acquired Coagulation Disorders and TTP
Fig. 8.1 Generation of cross-linked fibrin by thrombin and factor XIIIa following activation of coagulation, and consequences of plasmin degradation of cross-linked fibrin. (a) Following activation of coagulation, thrombin activates…
