Chapter Outline Investigation of membrane defects 229 Osmotic fragility as measured by lysis in hypotonic saline 229 Osmotic fragility after incubating the blood at 37 °C for 24 H 230…
Chapter Outline Sources of radioisotopes 352 Radiation protection 352 Apparatus for measuring radioactivity in vitro 353 Apparatus for measuring radioactivity in vivo 353 Measurement of radioactivity with a scintillation counter…
Chapter Outline Investigation of haemolytic anaemia 215 Plasma haemoglobin 216 Sample collection 216 Peroxidase method 216 Spectrophotometric method 216 Normal range 217 Significance of increased plasma haemoglobin 217 Serum haptoglobin…
Chapter Outline Tests for the acute-phase response 93 Erythrocyte sedimentation rate 94 Plasma viscosity 97 Whole blood viscosity 97 Heterophile antibodies in serum: diagnosis of infectious mononucleosis 97 Screening tests…
Chapter Outline Assessing the likelihood of acquired haemolytic anaemia 254 Assessment of the blood film and count in suspected acquired haemolytic anaemia 254 Immune haemolytic anaemias 255 Types of autoantibody…
Chapter Outline Methodologies 127 DNA extraction 127 Polymerase chain reaction 127 Amplification refractory mutation system (ARMS) 130 Gap-PCR 130 Fusion gene analysis 131 Restriction enzyme digestion 131 Allele-specific oligonucleotide hybridisation…
Chapter Outline Iron metabolism 166 Dietary iron absorption 166 Dietary and luminal factors 166 Iron absorption at the molecular level 167 Regulation of iron absorption 167 Cellular iron uptake and…
Chapter Outline Cobalamin absorption and metabolism 188 Folate absorption and metabolism 191 Haematological features of megaloblastic anaemia 193 Differential diagnosis of macrocytic anaemia 194 Metabolic insufficiency 194 Testing strategy for…
Chapter Outline Aspiration of the bone marrow 113 Consent and safety 113 Performing a bone marrow aspiration 113 Puncture of the ilium 114 Puncture of the sternum 114 Comparison of…
Chapter Outline Haemoglobinometry 19 Measurement of haemoglobin concentration using a spectrometer (spectrophotometer) or photoelectric colorimeter 19 Haemiglobincyanide (cyanmethaemoglobin) method 20 Diluent 20 Haemiglobincyanide reference standard 20 Method 21 Calculation of…
