Hemostasis Screening Assays
Fig. 5.1 Platelet satellitism and platelet clumping in a blood smear from a patient with EDTA-pseudothrombocytopenia (Courtesy Sherrie L. Perkins, MD, University of Utah Medical Center [Wright’s stain, oil immersion])…
Testing for Inherited and Acquired Thrombotic Disorders
Fig. 9.1 Regulation of thrombin activity by vascular endothelium. The effects of major antithrombotic properties of the blood vessel wall are shown. Regulation of thrombin activity is important because thrombin…
Testing for Inherited Bleeding Disorders
Disorders associated with: Thrombocytopenia Platelet dysfunction (qualitative platelet disorders) Coagulation disorders Autosomal recessive Autosomal recessive X-linked Bernard-Soulier syndrome Bernard-Soulier syndrome Factor VIII deficiency (hemophilia A) Congenital amegakaryocytic thrombocytopenia Glanzmann’s thrombasthenia…
Coagulation Testing and Transfusion Medicine
Fibrinogen deficient curve % Activity Fibrinogen, mg/dL PT (s) (normal 10.7–15.0 s) PTT (s) (normal 25–40 s) 100 288 11 28 80 230.4 11.4 29.8 70 201.6 11.6 29.8 40…
Pharmacogenetic Testing for Anticoagulant and Antiplatelet Therapies
CYP2C9 VKORC1 *1/*1 *1/*2 *1/*3 *2/*2 *2/*3 *3/*3 GG Typical Typical Moderate ↓ Moderate ↓ Moderate ↓ Significant ↓ AG Typical Moderate ↓ Moderate ↓ Moderate ↓ Significant ↓ Significant…
Collection of Coagulation Specimens
Hematocrit, % Blood volume (excluding citrate), mL 4.5 2.7 1.8 0.9 56 0.41 0.24 0.16 0.08 57 0.40 0.24 0.16 0.08 58 0.39 0.23 0.16 0.08 59 0.38 0.23 0.15…
Acquired Coagulation Disorders and TTP
Fig. 8.1 Generation of cross-linked fibrin by thrombin and factor XIIIa following activation of coagulation, and consequences of plasmin degradation of cross-linked fibrin. (a) Following activation of coagulation, thrombin activates…
Testing for Acquired Platelet Disorders
8. Kunz D, Kunz WS, Scott CS, et al. Automated CD61 immunoplatelet analysis of thrombocytopenic samples. Br J Haematol. 2001;112:584–92.PubMedCrossRef 9. De la Salle BJ, McTaggart PN, Briggs C, et…
Instrumentation for the Coagulation Laboratory
Manufacturer Instrument Clot detection method Compensation for interfering substances Instrumentation laboratories ACL series Photo-optical Interrogating wavelength Siemens healthcare diagnostics Sysmex series Photo-optical Blank measurement BCS Photo-optical Blank measurement Second wavelength…
Role and Responsibilities of the Laboratory Director
General responsibilities Personnel Facilities and safety Test procedures Quality management Consultation and education Communication Operational management Overall operation and administration Needs assessment Biosafety Test menu selection Quality program Accessible to…
