Chapter Outline The haemoglobin molecule 283 Structural variants of haemoglobin 284 Haemoglobins with reduced solubility 284 Unstable haemoglobins 285 Haemoglobins with altered oxygen affinity 285 Haemoglobin M 285 Thalassaemia syndromes…
Chapter Contents Management structure and function 512 Staff appraisal 512 Continuing professional development 513 Strategic and business planning 513 Test reliability 515 Test selection 516 Test utility 516 Instrumentation 517…
Chapter Outline Introduction to thrombophilia 410 Pre-analytical factors 411 Tests for the presence of a lupus anticoagulant 411 Dilute Russell’s viper venom time 412 Platelet neutralisation test 413 Kaolin clotting…
Chapter Outline Investigation of membrane defects 229 Osmotic fragility as measured by lysis in hypotonic saline 229 Osmotic fragility after incubating the blood at 37 °C for 24 H 230…
Chapter Outline Sources of radioisotopes 352 Radiation protection 352 Apparatus for measuring radioactivity in vitro 353 Apparatus for measuring radioactivity in vivo 353 Measurement of radioactivity with a scintillation counter…
Chapter Outline Investigation of haemolytic anaemia 215 Plasma haemoglobin 216 Sample collection 216 Peroxidase method 216 Spectrophotometric method 216 Normal range 217 Significance of increased plasma haemoglobin 217 Serum haptoglobin…
Chapter Outline Tests for the acute-phase response 93 Erythrocyte sedimentation rate 94 Plasma viscosity 97 Whole blood viscosity 97 Heterophile antibodies in serum: diagnosis of infectious mononucleosis 97 Screening tests…
Chapter Outline Assessing the likelihood of acquired haemolytic anaemia 254 Assessment of the blood film and count in suspected acquired haemolytic anaemia 254 Immune haemolytic anaemias 255 Types of autoantibody…
Chapter Outline Methodologies 127 DNA extraction 127 Polymerase chain reaction 127 Amplification refractory mutation system (ARMS) 130 Gap-PCR 130 Fusion gene analysis 131 Restriction enzyme digestion 131 Allele-specific oligonucleotide hybridisation…
Chapter Outline Iron metabolism 166 Dietary iron absorption 166 Dietary and luminal factors 166 Iron absorption at the molecular level 167 Regulation of iron absorption 167 Cellular iron uptake and…
