Keywords Adoptive immunotherapy, dendritic cells, electropermeabilization, gene delivery systems, gene editing, gene silencing, gene transfer, hematopoietic, lentiviral vectors, LTR, mesenchymal stem cells, mitochondrion gene therapy, pseudotyping, retroviral vectors, RNAi, RRE,…
Keywords Cancer, vaccines, poxviruses, TRICOM, immunotherapy, intratumoral vaccination, immunostimulatory molecules, costimulatory molecules In this chapter, we review the use of recombinant poxviral vectors containing transgenes for costimulatory molecules and…
Keywords FDA, CBER, cancer gene therapy, regulatory review, CMC, pharmacology/toxicology, clinical, drug development Introduction FDA’s Regulatory Responsibility and Gene Therapy Submissions In the United States, the U.S. Food and…
Keywords MGMT, P140K, chemoprotection, drug-resistance gene therapy, hematopoietic stem cell gene therapy, glioblastoma Introduction to MGMT Gene Transfer in Cancer Therapeutics The O 6 -methylguanine methyltransferase (MGMT) catalytic protein…
Keywords Sodium iodide symporter (NIS), viral vector, lentivirus, radioiodide, tumor-specific targeting promoter Sodium Iodide Symporter Thyroid hormone synthesis requires a variety of molecules in thyroid follicular cells, including sodium…
Keywords Ethics, translational research, preclinical studies, trial design, subject enrollment Ethics Beyond Research Regulation In this chapter, we address key ethical issues concerning the conduct of translational anticancer gene…
Keywords Gene therapy, hematopoiesis, cytidine deaminase, doxycycline, miRNA, Ara-C Background Transfer of Drug-Resistance Genes into Hematopoietic Stem Cells Given their ability to self-renewal, proliferate extensively, and differentiate into all…
Keywords Salmonella , genetic engineering, gene delivery, gene therapy, attenuation, toxicity, imaging Introduction Gene therapy is considered to be one of the most promising therapeutic approaches for treating cancer,…
Keywords IL-12, adenovirus, RheoSwitch®, activator ligand, dendritic cells, controlled expression, intratumoral, immunotherapy Introduction Tumors grow aggressively, are invasive, and may spread to distant metastatic sites, not only because of…
Keywords Gene therapy, drug resistance, glioblastoma, chemoprotection, in vivo selection, MGMT, hematopoietic stem cell, temozolomide Introduction Gene therapy was initially developed as an ideal treatment for defective single-gene diseases…
