The same lack of technical improvements applies to various different steps along the histopathological processing. Tissue fixation is still conducted manually and with the use of formaldehyde originally proposed by…

Fig. 1 Morphological evaluation of two differently processed parts of a bone marrow trephine biopsy (H&E). a, c Fixation in 10 % neutral buffered formalin (NBF) at room temperature (RT) for at…

Fig. 1 ×200 image of a 4-mm-thick human tonsil section placed directly into 40 °C 10 % neutral buffered formalin for 1 h. Sample is stained with hematoxylin and eosin to reveal tissue morphology…

Rapid MW protocol logos™ (1 h 39 min) Reagent Time Phase T °C Pressure (mBar) Formalin 10 % NBF 25′ 1 = 10′ 50°    2 = 15′   Ethanol 60° 2′    Ethanol 99° 3′    Ethanol 99° 16′ 1 = 8′ 65°   …

Indications and contraindications for a frozen section in mammapathology  Applicable Non-applicable Clarification of dignity in preoperative uncertain situations (e.g., multicentricity) +  Clarification of representativeness (dense breast parenchyma, dislocation of wire…

Fig. 1 Sequence of the pre-analytical steps. From the surgical theaters, tissue specimens are transferred to Pathological Anatomy. Grossing is immediately followed by fixation and paraffin embedding. After sectioning of the…

Tumor location Method Material Central tumor with bronchial contact Bronchoscopy Conventional bronchial biopsy Bronchial cryobiopsy Brush cytology Bronchoalveolar lavage Peripheral tumor Bronchoscopy  Transbronchial biopsy Transbronchial cryobiopsy Transbronchial needle aspiration Brush…

Fig. 1 Flowchart of processes in the acquisition of specimens prior to sample analysis. This study focuses on sampling of tissue specimens fresh-frozen with entry at pathology Biorepositories, tissue, and sample…