GENE STRUCTURE
GENE STRUCTURE Part of “CHAPTER 3 – BIOSYNTHESIS AND SECRETION OF PEPTIDE HORMONES“ Polypeptide hormones may be encoded by single or multiple genes. Frequently, a hormone requires only intramolecular folding…
OVERVIEW OF PEPTIDE HORMONE SYNTHESIS AND SECRETION
OVERVIEW OF PEPTIDE HORMONE SYNTHESIS AND SECRETION Part of “CHAPTER 3 – BIOSYNTHESIS AND SECRETION OF PEPTIDE HORMONES“ Proteins are important as the backbones of polypeptide hormones and as integral…
BIOSYNTHESIS AND SECRETION OF PEPTIDE HORMONES
BIOSYNTHESIS AND SECRETION OF PEPTIDE HORMONES William W. Chin In the endocrine system, hormones are factors produced by groups of cells clustered in specific tissues, commonly known as glands, and…
FUTURE PROSPECTS
FUTURE PROSPECTS Part of “CHAPTER 2 – MOLECULAR BIOLOGY: PRESENT AND FUTURE“ HUMAN GENOME PROJECT The Human Genome Project was begun in 1990 by the U.S. government and is coordinated…
STRATEGIES FOR MAPPING GENES ON CHROMOSOMES
STRATEGIES FOR MAPPING GENES ON CHROMOSOMES Part of “CHAPTER 2 – MOLECULAR BIOLOGY: PRESENT AND FUTURE“ GENETIC LINKAGE MAPS AND QUANTITATIVE TRAIT LOCI A genetic linkage map shows the relative…
DNA ARRAYS FOR THE PROFILING OF GENE EXPRESSION
DNA ARRAYS FOR THE PROFILING OF GENE EXPRESSION Part of “CHAPTER 2 – MOLECULAR BIOLOGY: PRESENT AND FUTURE“ Two variants of DNA-array chip design exist.20,21 The first consists of cDNA…
GENETIC MANIPULATIONS IN ANIMALS IN VIVO
GENETIC MANIPULATIONS IN ANIMALS IN VIVO Part of “CHAPTER 2 – MOLECULAR BIOLOGY: PRESENT AND FUTURE“ TRANSGENIC APPROACHES To create transgenic mice, DNA is injected into the male pronucleus of…
APPROACHES TO THE QUANTITATIVE ASSESSMENT OF GENE EXPRESSION
APPROACHES TO THE QUANTITATIVE ASSESSMENT OF GENE EXPRESSION Part of “CHAPTER 2 – MOLECULAR BIOLOGY: PRESENT AND FUTURE“ TRANSCRIPTION ASSAYS Nuclear Run-On Assays. Several assays are available that provide an…
GENE AMPLIFICATION BY POLYMERASE CHAIN REACTION
GENE AMPLIFICATION BY POLYMERASE CHAIN REACTION Part of “CHAPTER 2 – MOLECULAR BIOLOGY: PRESENT AND FUTURE“ The development of the polymerase chain reaction PCR, a technique for the rapid amplification…
GENOMIC LIBRARIES AND GENE ISOLATION
GENOMIC LIBRARIES AND GENE ISOLATION Part of “CHAPTER 2 – MOLECULAR BIOLOGY: PRESENT AND FUTURE“ Southern Blots and Hybridization Screening. The techniques used in the cloning of genomic DNA are…
