Assay for Cortisol: Chemiluminescence Immunoassay (CLIA) . Precautions: None. Interpretation: Serum cortisol concentration more than 1.4 mcg/dl at 15 min after Combined Dex-CRH test is suggestive of Cushing’s syndrome [1]. Caveats: We…
Assay for 17 OHP: Radioimmunoassay (RIA) . Interpretation: With late onset 21-hydroxylase deficiency, the absolute value of 17-hydroxyprogesterone at 60 min sample is > 10 ng/dl [2, 3]. Caveats: Baseline androgen levels return…
Assay for GH: Immunoenzymatic Precautions: Nausea (30 %), vomiting/retching (10 %), and headaches (10 %) may occur during and after the test. (Administration of intravenous antiemetics can be considered.) Late hypoglycemia may rarely…
Assay for Cortisol: Electrochemiluminescence immunoassay (ECLIA) . Precautions: Cosyntropin is category C of pregnancy. Interpretation: Normal response: Peak stimulated cortisol value > 1.2 mcg/dl [3, 4] at 30 or 60 min. Most…
50 mg of captopril. Blood tubes for plasma renin activity (PRA) and aldosterone. Assay for PRA and Aldosterone: Radioimmunoassay. Precautions: Monitor BP hourly. Interpretation: Plasma aldosterone is normally suppressed by captopril…
Confirm the medication doses with physician. Assay for GH: GH:Immunoenzymatic Assay Precautions: Facial flushing occurs immediately after administration of GHRH in about half of the patients. Paresthesias, nausea, and abnormal…
Precautions: Sustained hypotension may occur. Keep patient supine throughout testing. Patients may experience dry mouth or drowsiness [1] Interpretation: Criteria for diagnosis of pheochromocytoma : 3 h after clonidine; one of…
Assay for Aldosterone: Enzymatic Radioimmunoassay (RIA). Precautions: Do not perform this test in patients with severe uncontrolled hypertension, renal insufficiency, cardiac insufficiency, cardiac arrhythmia, or severe hypokalemia [1]. Interpretation: With…
Sample ACTH (pg/mL) Prolactin (ng/mL) Cortisol (µg/mL) Petrosal sinus Petrosal sinus Peripheral Right Left ACTH ratio Peripheral Right Left Prolactin ratio Peripheral R/P L/P R/L R/P L/P ADH (− 10 min) ADH…
Assay for Cortisol: Chemiluminescence immunoassay (CLIA) . Precautions: Cosyntropin is category C of pregnancy. Interpretation: Normal response: Peak stimulated cortisol value > 18 mcg/dl at 30 min [1, 2]. Most patients achieve higher…
