MEASUREMENT OF VITAMIN D METABOLITES



MEASUREMENT OF VITAMIN D METABOLITES





Part of “CHAPTER 54 – VITAMIN D“



METHODS

The measurement of vitamin D in biologic fluids was pioneered with bioassays that measured either the linear calcification rate (line test) or a rise in blood calcium concentration (calcemic assay) in rats after the administration of vitamin D or its 25(OH)D analog. Modern assays, which are less tedious and more specific, use radioligand-binding techniques, physico-chemical methods, or a combination of these.59



25-HYDROXYVITAMIN D

In the early 1970s, the first competitive protein-binding assays for 25(OH)D were developed.60,61 This method uses the vitamin D transport protein as a specific binder. Extraction and chromatographic purification of 25(OH)D generally are required before the binding assay can be conducted. It also is possible to measure 25(OH)D by high-pressure liquid chromatography and UV absorbance spectroscopy.

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Aug 25, 2016 | Posted by in ENDOCRINOLOGY | Comments Off on MEASUREMENT OF VITAMIN D METABOLITES

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