Non-Hodgkin lymphomas
These are a large group of clonal lymphoid tumours, about 85% of B cell and 15% of T or NK (natural killer) cell origin (Table 20.1). Their clinical presentation and natural history are more variable than in Hodgkin lymphoma. They are characterized by an irregular pattern of spread and a significant proportion of patients develop extranodal disease. Their frequency has increased markedly over the last 50 years and with an incidence of approximately 17 in 100 000 they now represent the fifth most common malignancy in some developed countries (see Fig. 11.11). The aetiology of the majority of cases of non-Hodgkin lymphomas (NHL) is unknown although infectious agents are an important cause in particular subtypes (Table 20.2). There are also considerable geographical variation (Table 20.2). The World Health Organization (WHO) classification also recognizes age (paediatric or elderly) and site of involvement (e.g. skin, central nervous system (CNS), intestine, spleen, mediastinal) as important in disease classification.
Mature B-cell neoplasms | Mature T-cell and NK-cell neoplasms |
Chronic lymphocytic leukaemia/small lymphocytic lymphoma | T-cell prolymphocytic leukaemia |
T-cell large granular lymphocytic leukaemia | |
B-cell prolymphocytic leukaemia | Adult T-cell lymphoma/leukaemia |
Splenic marginal zone lymphoma | Extranodal NK/T-cell lymphoma, nasal type |
Hairy cell leukaemia | Enteropathy-associated T-cell lymphoma |
Lymphoplasmacytic lymphoma–Waldenström macroglobulinaemia | Mycosis fungoides |
Sézary syndrome | |
Heavy chain diseases | Peripheral T-cell lymphoma |
Plasma cell myeloma | Angioimmunoblastic T-cell lymphoma |
Plasmacytoma | Anaplastic large cell lymphoma, ALK positive |
Extranodal marginal zone lymphoma of mucosa-associated lymphoid tissue (MALT lymphoma) | |
Follicular lymphoma | |
Mantle cell lymphoma | |
Diffuse large B-cell lymphoma | |
Burkitt lymphoma |
ALK, anaplastic lymphoma kinase, the gene on chromosome 2 which is overexpressed; NK, natural killer.
Infection | Tumour |
Virus | |
HTLV–1 | Adult T-cell leukaemia/lymphoma |
Epstein–Barr virus | Burkitt and Hodgkin lymphomas; PTLD |
HHV–8 | Primary effusion lymphoma; multicentric Castleman’s disease |
HIV–1 | High-grade B-cell lymphoma, primary CNS lymphoma, Hodgkin lymphoma |
Hepatitis C | Marginal zone lymphoma |
Bacteria | |
Helicobacter pylori | Gastric lymphoma (MALT) |
Protozoa | |
Malaria | Burkitt lymphoma |
HHV-8, human herpes virus 8; HIV, human immunodeficiency virus; HTLV-1, human T-lymphotropic virus type 1; MALT, mucosa-associated lymphoid tissue; PTLD, post-transplant lymphoproliferative disease.
Classification
The lymphomas are classified within a group of mature B-cell and T-cell neoplasms, which also includes some chronic leukaemias and myeloma which are described in Chapters 18 and 21, respectively (Table 20.1). In this chapter we consider the more common lymphoma subtypes within this classification.
Cell of origin
The normal B-cell development stages are illustrated in Fig. 9.10. B-cell lymphomas tend to mimic normal B cells at different stages of development (Fig. 20.2). They can be divided into those resembling precursor (bone marrow) B cells, those resembling germinal centre (GC) cells and those post-GC cells in lymph nodes. T-cell lymphomas resemble precursor T cells in bone marrow or thymus, or peripheral mature T cells.
Low-and high-grade non-Hodgkin lymphomas
The NHL are a diverse group of diseases and vary from highly proliferative and potentially rapidly fatal diseases to some of the most indolent and well-tolerated malignancies. For many years clinicians have subdivided lymphomas into low-and high-grade disease. This approach is valuable as, in general terms, the low-grade disorders are relatively indolent, respond well to chemotherapy but are very difficult to cure whereas high-grade lymphomas are aggressive and need urgent treatment but are more often curable.
Leukaemias and lymphomas
The difference between lymphomas, in which lymph nodes, spleen or other solid organs are involved, and leukaemias, with predominant bone marrow and circulating tumour cells, may be blurred. A single lymphoproliferative disease (e.g. chronic lymphocytic leukaemia and small lymphocytic lymphoma) merge with each other with the identical cell genotype and immunophenotype. Lymphoma cells may circulate (e.g. in follicular, mantle cell, diffuse large B-cell lymphomas and the Sézary syndrome). B-cell acute lymphoblastic leukaemia (B-ALL) and T-ALL and the corresponding B-and T-lymphoblastic lymphomas are different manifestations of the same diseases and are usually treated in identical fashion.
Clinical features of non-Hodgkin lymphomas
1 Superficial lymphadenopathy The majority of patients present with asymmetric painless enlargement of lymph nodes in one or more peripheral lymph node regions.
2 Constitutional symptoms Fever, night sweats and weight loss occur less frequently than in Hodgkin lymphoma and their presence is usually associated with disseminated disease.
3 Oropharyngeal involvement In 5–10% of patients there is disease of the oropharyngeal lymphoid structures (Waldeyer’s ring) which may cause complaints of a ‘sore throat’ or noisy or obstructed breathing.
4 Symptoms due to anaemia, infections due to neutropenia or purpura with thrombocytopenia may be presenting features in patients with diffuse bone marrow disease. Cytopenias may also be autoimmune in origin or due to sequestration in the spleen. Infections may occur as a result of neutropenia or reduced cell immunity (e.g. herpes zoster).
5 Abdominal disease The liver and spleen are often enlarged and involvement of retroperitoneal or mesenteric nodes is frequent. The gastrointestinal tract is the most commonly involved extranodal site after the bone marrow, and patients may present with acute abdominal symptoms.
6 Other organs Skin, brain, testis or thyroid involvement is not infrequent. The skin is also primarily involved in two unusual, closely related T-cell lymphomas, mycosis fungoides and Sézary syndrome.
Investigations
Histology
Lymph node biopsy or trucut biopsy of lymph node or of other involved tissue (e.g. bone marrow or extranodal tissue) is the definitive investigation (Figs 20.3 and 20.4). Morphological examination is assisted by immunophenotypic and, in some cases, genetic analysis (Table 20.3). For B-cell lymphomas, expression of either κ or λ light chains confirms clonality and distinguishes the disease from a reactive node (Fig. 20.5). A fine needle aspiration may be performed to exclude another cause of lymphadenopathy (e.g. tuberculosis, carcinoma) but is not useful in establishing a diagnosis of lymphoma.
CLL, chronic lymphocytic leukaemia; MALT, mucosa – associated lymphoid tissue.
More detailed cytogenetics are given in Table 11.1 .
Laboratory investigations
1 In advanced disease with marrow involvement there may be anaemia, neutropenia or thrombocytopenia (especially if the spleen is enlarged or there are leucoerythroblastic features).
2 Lymphoma cells (e.g. mantle zone cells, ‘cleaved follicular lymphoma’ or ‘blast’ cells) may be found in the peripheral blood in some patients (Fig. 20.6). 3 HIV should be tested for in all patients.
4 Trephine biopsy of marrow is valuable (Fig. 20.7). Paradoxically, bone marrow involvement is found more frequently in low-grade malignant lymphomas.