The mucosal immune system of the GI tract contains unique cell subsets that are structured in such a way as to favor the coexistence of the host with its flora, support food absorption, as well as induction of effector responses to pathogens.₁ The primary barrier preventing transit of microorganisms and large molecules from the environment into the body is a simple epithelium made up of a single layer of cells overlaid with mucus and connected by junctional complexes. The gutassociated lymphoid tissue (GALT) can be divided into effector sites consisting of lymphocytes scattered throughout the epithelium and lamina propria (LP) of the mucosa and organized inductive sites. These inductive sites include the largest lymph node of the body, the mesenteric lymph node (MLN), Peyer patches, as well as cryptopatches and isolated lymphoid follicles (ILFs) that are distributed along the wall of the small and large intestine.¹ ILFs are inducible structures recently described in both mice and humans that develop after birth in response to environmental signals, such as those derived form the microbiota.^2,3,4 Peyer patches are macroscopic lymphoid aggregates consisting of large B-cell follicles with interspersed T-cell areas found in the submucosa along the length of the small intestine. In these structures, the lymphoid areas are separated from the intestinal lumen by a single layer of epithelial cells known as the follicle-associated epithelium and a more diffuse area below the epithelium known as the subepithelial dome. Notably, the follicle-associated epithelium contains unique cells referred to as microfold cells that have the capacity to transport organisms and particles from the gut lumen across the epithelial barrier.⁵ These cells also represent a major point of pathogen entry.⁵

A central strategy utilized by the mucosal immune system to maintain its homeostatic relationship with the microbiota and limit pathogen exposure is to minimize contact between luminal microorganisms and the epithelial cell surface. This is accomplished by promoting a physical barrier through the production of mucus, immunoglobulin (Ig)A, and antimicrobial proteins. All intestinal cell lineages, including enterocytes, goblet cells, and Paneth cells, can produce antimicrobial peptides. These molecules play a significant role in the control of pathogenic infections as well as in limiting exposure to the commensal microbiota. Epithelial cell-derived antimicrobial proteins are members of a diverse family including defensins, cathelicidins, and C-type lectins.⁶ Some of these molecules, such as &agr;-defensins, are constitutively expressed. In other cases, engagement of pattern recognition receptors (PRRs) by commensally derived products induce the expression of a variety of antimicrobial peptides, which are critical to prevent translocation of gut bacteria across mucosal barriers.⁶ These proteins can exert antimicrobial functions by direct killing resulting from enzymatic attack of the bacterial cell wall or by
disrupting the bacterial inner membrane.⁶ Additionally, some antimicrobial proteins can function by depriving bacteria of essential heavy metals, such as iron.⁷ One of the best characterized mucosal antimicrobial peptides is RegIII&ggr;. This lectin is expressed soon after birth or following colonization of germfree mice.⁸ Production of RegIII&ggr; is tightly controlled by the flora in an MyD88-dependent manner and has a direct microbicidal effect on gram-positive bacteria.^8,9,10 Similarly, the PRR nucleotide-binding oligomerization domain-containing protein (NOD) 2 controls expression of a subset of &agr;-defensins and cryptdins by Paneth cells.¹¹ Critically, antimicrobial proteins are retained in the mucus layer and are virtually absent from the luminal content.¹² Such a feature allows antimicrobial activity to be concentrated within a region bordering the epithelial cell layer. Recent findings demonstrate that the accumulation of RegIII&ggr; in the mucus contributes to the maintenance of the segregation between the microbiota and the host intestine.¹³

The stratified mucus layer plays a crucial role in the maintenance of the physical segregation between the microbiota and host tissue, as well as preventing pathogen invasion and supporting their clearance. In particular, the mucus layer limits commensal and pathogenic bacterial contact with the epithelium preventing transport of bacteria to distal sites.¹⁴ To date,¹⁷ mucus (MUC) genes have been described,⁷ of them coding for secreted mucins (MUC2, MUC5A, MUC5B, MUC6, MUCC19, MUC7, and MUC8) that are differentially expressed in various mucosal compartments.¹⁴ The secreted, gel-forming, mucin glycoproteins that constitute the major macromolecular component of mucus are produced by goblet cells found throughout the GI tract and airways. In the airways, both mucus cells and serous cells produce defined mucins.¹⁵ Paneth cells represent another major secretory cell type within the GI tract.¹⁴ Mucus is found in the entire GI tract but varies in thickness ranging from 700 &mgr;m in the stomach and large intestine to 150 to 300 &mgr;m in the small intestine.¹⁶ The inner layer of the colonic mucus is attached to the epithelium and has a stratified appearance, ranging in thickness from 50 to 100 &mgr;m. Based on its structural capacity, this layer acts as a filter and is impermeable to commensal bacteria.^17,18,19 The inner mucus layer is renewed by goblet cell secretion and at its luminal border is converted into an outer mucus layer. This outer layer expands in volume allowing commensal bacteria access to this zone.¹⁸ Contrasting the large intestine, the small intestine is only covered by a single layer of mucus that is not attached to the epithelium and more permeable to bacteria.^16,20 Of note, commensal bacteria have a high proportion of their genome devoted to production of enzymes involved in glycan degradation and are able to utilize the released mucin monosaccharides as energy sources.^21,22

More than 40 years ago, Peyer patches were identified as the major source of IgA-producing B cells.¹⁶ IgA can also be generated in regional lymph nodes as well as in ILFs.⁴⁵ The diffuse tissue of the LP can also support IgA production in the absence of follicular structures.^46,47,48 In order to express IgA, B cells must undergo class switch recombination in which deoxyribonucleic acid (DNA) is spliced out of the heavy chain constant region loci to replace the &mgr;/&dgr; genes with the &agr; gene segment downstream of the recombined VDJ segment. The GC-rich splice sites upstream of the gene segment for each Ig constant region are the targets for enzymes such as activation-induced (cytidine) deaminase (AID), which catalyze the reaction sequence. In humans, there are two C&agr; regions generating the two IgA isotypes, IgA1 and IgA2.^30,31,32 Following induction in the intestinal lymphoid structures, B cells recirculate through the mesentery, lymphatic systems, and the bloodstream to home back to the intestinal mucosa where they develop into IgA-secreting plasma cells. Secretory IgA is produced as a result of cooperation between plasma cells in the LP that secrete dimeric IgA and epithelial cells that transport IgA into the lumen. This mechanism of transport involves covalent binding of dimeric IgA to the polymeric Ig receptor, which then carries it within vesicles through intestinal epithelial cells to the luminal surface. At this point, IgA is released by proteolytic cleavage of the polymeric Ig receptor leaving a fragment of the transport protein (termed secretory component) that remains associated with the joining chain of the IgA dimer forming a secretory IgA complex with noninflammatory protective functions.^49,50,51 Indeed, secretory IgA can bind to bacteria without activating complement or stimulating the release of inflammatory mediators by innate cells.^52,53

Mucosal IgA can recognize antigen with high- and lowaffinity binding modes.⁵⁴ Generally, high-affinity IgA neutralizes microbial toxins and invasive pathogens whereas low-affinity IgA confines commensals to the lumen.³² Highaffinity IgA is believed to emerge from follicular B cells stimulated in a T cell-dependent manner, whereas low-affinity IgA can develop from extrafollicular B cells stimulated in a T cell-independent fashion.⁵⁵ However, such distinction of origin and function is not absolute.⁵⁶ Antigen at mucosal sites can stimulate IgA responses through T cell-dependent pathways that are initiated in mucosal lymphoid structures such as Peyer patches and MLN.⁵⁴

Various mechanisms can promote the induction of IgA in a T cell-independent manner. In particular, transforming growth factor (TGF)-&bgr; that is highly enriched at mucosal surfaces controls B-cell responsiveness and promotes class switch recombination to IgA, in combination with interleukin (IL)-2 and IL-10 that act synergistically.⁵⁷ Mechanistically, TGF-&bgr;1 binds to the TGF-&bgr; receptor complex that has a sterile transcript for the I&agr; locus upstream of the S&agr; recombination segment. Such a phenomenon is associated with signaling via mothers against decapentaplegic-2, -3, and -4 that bind to the I&agr; promoter in combination with the runt DNA binding factor RUNX3.^{58,59,60,61,62,63,64} A cytokine of the tumor necrosis factor (TNF) ligand family referred to as B cell-activating factor (BAFF) and a proliferation-inducing ligand (APRIL) can also induce NF-&kgr;B activation and IgA induction.^65,66 Further, as will be described in more detail later in this chapter, synthesis of the vitamin A metabolite retinoic acid (RA) by GALT dendritic cells (DCs) is crucial for the generation of mucosal IgA.^67,68

IgA plays a major homeostatic role by controlling host interactions with the microbiota as well as a protective role against pathogens.^6,69 Although nonspecific IgA can limit epithelial invasion, pathogen-specific IgA plays an important role in the control of mucosal infections. For example, IgA production has been shown to control Shigella flexneri during infection by binding the bacteria at the mucosal layer,³⁷ whereas IgA against the O-antigen component of lipopolysaccharides can protect against Salmonella infection.⁷⁰ IgA can also shape the microbiota composition, as demonstrated in mice lacking IgA that have expansion of segmented filamentous bacteria (SFB) and other Clostridium-related species in the intestinal tract.⁷¹ In the context of IgA-microbiota dialogue, IgA responses lack typical memory characteristics and respond rapidly to changes in gut flora composition. This point was demonstrated using reversible colonization of mice with a strain of auxotrophic Escherichia coli that required nutrients unavailable from mammalian host metabolites.⁷² IgA responses persisted in the absence of bacteria; however, introduction of additional species of bacteria caused a rapid decline in IgA specific for the auxotrophic E. coli.⁷² As the microbiota composition can fluctuate in the context of infection or due to dietary changes, such a strategy would promote a dynamic IgA repertoire, allowing IgA to swiftly adapt to the commensals present at a given time.⁷²

In the small intestine, IELs are highly abundant and are essentially composed of TCR&agr;&bgr;+ or TCR&ggr;&dgr;+ CD8+ T cells. In contrast, in the large intestine the frequency of IELs is reduced and mostly represented by TCR&agr;&bgr; CD4+ T cells.^74,76,77,78 Although the development and origin of the various IELs is the subject of long debate, all IEL subsets are progeny of bone marrow precursors that initially develop in the thymus.⁷⁹ Some IEL precursors go through alternative self-antigen-based thymic maturation processes that lead to the functional maturation of CD4 and CD8&agr;&bgr; double negative, TCR&agr;&bgr;-expressing or TCR&ggr;&dgr;-expressing T cells that directly migrate to the intestinal epithelium.^80,81,82 The capacity of IELs to home to the intestinal epithelium is controlled by the expression of CCR9 on the surface of IEL precursors and its ligand CCL25.^83,84 IELs also express the integrin &agr;E&bgr;7 (CD103) that can interact with E-cadherin on epithelial cells.⁸⁵ Additionally, the mucosal environment leads to the induction of IELs from conventional TCR&agr;&bgr;+ cells that have matured as antigen-experienced cells in response to cognate antigen.⁷⁵ In particular TGF-&bgr;, highly enriched in the gut, leads to the development of TCR&agr;&bgr;+CD8&agr;&agr;+ IELs and the coexpression of CD8&agr; in conventional CD4+ T cells.⁸⁶ IEL repetoire is polyclonal at birth and in response to exogenous stimuli, including luminal antigens and microbial composition, these cells expand to become oligoclonal in the adult.⁸⁷ The development and expansion of IELs is also highly controlled by the cytokine IL-15 and by dietary metabolites. In particular, vitamin D and the arylhydrocarbon receptor, whose ligands are metabolized from the vegetable-derived phytochemical indile-3-carbinol, are required for the proper homeostasis of these cells.^75,88,89 Although mice and human IELs share a number of features, some notable differences exist in terms of their composition and phenotype⁹⁰ (see Table 34.1).

IELs have complex functions that include the promotion of local immunity concomitant with the maintenance of barrier integrity. Moreover, a role for IELs in promoting host defense has been revealed in various models of mucosal infection. For instance, TCR&agr;&bgr;+ CD8&agr;&bgr;+ IELs can promote effector responses to a variety of pathogens such as Eimeria vermiformis, Encephalitozoon cuniculi, and Toxoplasma gondii.^91,92,93,94 In the context of bacterial or parasitic infections, IELs can mediate pathogen clearance via their capacity to produce various inflammatory mediators such as interferon (IFN)&ggr; and TNF-&agr;.⁹⁵ IELs can also promote immunity to nematodes by releasing cytokines such as IL-13 that can promote mucus formation.⁹⁶ Moreover, IELs express various natural killer (NK) receptors, endowing them with a strong cytolytic activity leading to the direct elimination of damaged or infected cells.⁷⁵ Additionally, their protective role can result from the capacity of these cells to maintain the integrity of tight junctions between epithelial cells, therefore preventing epithelial transmigration of pathogens.⁹⁷ In this regard, IELs have been associated with the maintenance of barrier function in the context of simian immunodeficiency virus (SIV) or human immunodeficiency virus (HIV).^98,99 IELs can also exert regulatory function at mucosal sites. In particular, TCR&ggr;&dgr; IELs play a major anti-inflammatory role via their capacity to promote tissue repair upon injury and to release an array of factors with regulatory properties. In particular, &ggr;&dgr; T cells produce large amounts of keratinocyte growth factor, which plays an important role in supporting epithelial integrity.^100,101 Further, these cells can abundantly produce the immunoregulatory cytokines IL-10 and TGF-&bgr; that can both limit inflammatory responses at mucosal sites.^102,103,104 In mice, TCR&ggr;&dgr; IELs express predominantly V&ggr;5 and V&ggr;1, whereas in humans TCR&ggr;&dgr; IELs express essentially the V&ggr;1 gene segment.³⁸ To date, little is known about the specificity of these cells.

Although the role of IELs is to maintain mucosal homeostasis, various lines of evidence suggest that in defined settings, IELs can also contribute to tissue damage and inflammation. For instance, the number of TCR&ggr;&dgr;+ cells correlates with disease severity in inflammatory bowel disease (IBD) patients and, experimentally, these cells have been shown to promote mucosal inflammation.^{105,106,107,108} Further, CD8&agr;&bgr;+ IELs can also contribute to the initiation of experimental colitis. More particularly, IELs have been associated with the exacerbation of celiac disease, an inflammatory disorder in genetically susceptible individuals associated with the induction of inflammatory T-cell responses against dietary gluten.⁹⁰ Celiac disease is characterized by a dramatic increase of the IEL compartment.⁹⁰ TCR-activated CD8&agr;&bgr;+ TCR&agr;&bgr;+ IELs cause severe villous atrophy by targeting intestinal epithelial cells that express stress-induced major histocompatibility complex class I polypeptiderelated sequence antigen in an NKG2D-dependent manner.^109,110 Additionally, IL-15, a factor that is overexpressed in various mucosal disorders including celiac and Crohn diseases,⁹⁰ can trigger potent cytotoxic responses by CD8 &agr;&bgr;+ IELs via a NKG2D-DAP10-dependent signaling pathway.¹⁰⁹ Supporting a role for IELs in celiac disease pathogenesis, genome-wide association studies have identified a number of genes involved in the development, migration, and cytotoxic/NK function of IELs.¹¹¹

Immune reactivity against nonpathogenic gut elements is not only wasteful but is also known to lead to severe tissue damage. However, the development of active immunity is required to protect the host against invasive pathogens. This tight equilibrium is under the control of a complex network of mucosal mononuclear phagocytes (MPs) with unique properties (Fig. 34.1). Mucosal MPs form an organized cellular network in the LP and are distributed throughout the GALT.¹¹² In the LP, major histocompatibility complex II^hi CD11c^hi MPs can be subdivided into two main populations defined as CD103+CD11b+CX3CR1- and CD103-CD11b+CX3CR1+. Although some debate exists in the
literature, the current consensus is that CD103+ cells are mucosal DCs with capacity to rapidly migrate into lymphoid structures, whereas the CX3CR1+ populations are considered to be tissue resident macrophages.¹¹³ The gut also contains a minor population of CD103+CD11b-CD8&agr;+CX3CR1-DCs believed to be associated with ILFs.¹¹⁴ Additionally, the gut is home to plasmacytoid DCs.¹¹⁵ APC subset distribution varies anatomically with CD103+CD11b+ DCs mostly represented in the duodenum and CD103-CD11b+ DCs enriched in the large intestine.¹¹⁶

In the intestine, DC-restricted progenitors (termed common DC precursors) and pre-DCs give rise to CD103+CD11b+ and CD103+CD11b- populations.^113,117 In contrast, CD103-CD11b+ cells are monocyte derived.^113,117 Various cytokines, including FMS-like tyrosine kinase 3 ligand, granulocyte macrophage-colony stimulating factor, and macrophagecolony stimulating factor, control MP development and homeostasis.¹¹⁸ In the gut, these cytokines have a differential effect on the various MP subsets. Flt3L controls the differentiation of CD103+ DC (CD11b+ and CD11b-), granulocyte macrophage-colony stimulating factor the differentiation of CD103+CD11b+ DCs, and macrophage colony-stimulating factor receptor (M-CSFR) controls the homeostasis of CD103-CD11b+ cells.^113,117,119 The fractalkine receptor CX3CR1 also controls the development of CD103-CD11b+ CX3CR1+ cells, likely via its capacity to control the recruitment of progenitors to the tissue.¹²⁰ A number of transcription factors have been associated with DC lineage specification.¹²¹ In the gut, CD103+CD11b- DCs are highly dependent upon the transcription factors Id2, Irf8, and Batf3, whereas CD103+CD11b+ DCs are Notch2 dependent.^{113,122,123,124}

Luminal antigens, including macromolecules, bacteria, and viruses, gain access to the cells of Peyer patches and ILFs through microfold cells, present in the follicle-associated epithelium.^125,126,127 Microfold-cell transport is promiscuous and mediated by binding to surface-expressed carbohydrates in regions free of overlying mucus, but can be enhanced by the presence of antigen-specific IgA by immune targeting with anti-M-cell antibodies^128,129 or by oral administration of toll-like receptor (TLR)2 or TLR4 ligands.¹³⁰ A second site for antigen entry into the intestine is the nonfollicular absorptive epithelium, where both soluble
antigens and bacteria can gain access to DCs in the LP. This can occur by trans- or paracellular transport or by receptormediated trafficking, such as occurs through the neonatal FcR expressed on absorptive epithelial cells in humans.¹³¹ Luminal antigens can also be directly sampled by mucosal DCs. Indeed, subepithelial DCs can penetrate the epithelium monolayer by extending dendrites into the lumen, allowing them to sample particles and bacteria.^132,133,134 In the terminal ileum, this process is tightly dependent on the fractalkine receptor CX3CR1 and controlled by the microbiota in an MyD88-independent manner.^132,133,135 In addition to CX3CR1, CCL20 can also contribute to transepithelial dendrite formation.¹³³ Luminal antigen can gain access to mucosal sites by direct damage to the epithelium, as can occur during IBD or in response to infection with HIV¹³⁶ or Shigella flexneri.¹³⁷ Antigen sampling may also occur by uptake of exosomes from epithelial cells or across villous microfold cells.¹³⁸ Even in the absence of infection or inflammation, LP DCs constitutively traffic to MLN,¹³⁹ which appears to be a relatively active process. These migratory DCs can carry self- or cell-associated antigens from apoptotic epithelial cells¹⁴⁰ or soluble proteins given orally.¹⁴¹ Soluble antigens given orally can be processed by LP DCs, which then migrate to the MLN in a CCR7-dependent manner.¹⁴² Various lines of evidence support the idea that CD103+ DCs have enhanced migratory capacity to lymphoid structures compared to other mucosal MP subsets. Indeed, CD103+ DCs are highly represented in the gut afferent lymphatics,¹¹⁹ constitutively migrating to the MLN at steady state, and are the first to reach the MLN under inflammatory conditions.^113,119 In response to inflammation, various signals including lipopolysaccharides and flagellin can influence the capacity of mucosal DCs to transport intestinal commensals and pathogenic bacteria to the MLN.^143,144 A remarkable feature of this process is that commensal loaded LP DC migration is restricted to the mucosal immune compartment by the MLN.¹⁴³ Such a process is believed to contribute to the compartmentalization of mucosal immune responses. Although as further discussed, mucosal MP can promote the induction of tolerance under steady-state conditions, the function of these cells is highly contextual, and under inflammatory settings mucosal MP can rapidly adopt a highly inflammatory phenotype and induce effector responses.¹⁴⁵

Vitamin A is a fat-soluble essential nutrient obtained from foods containing vitamin A precursors (ie, carotenoids) or vitamin A itself in the form of retinyl esters.^154,155 Following absorption and arrival into circulation, retinyl esters enter the liver, where most of the vitamin A in the body is stored (Fig. 34.2).¹⁵⁶ Liver retinyl esters are continually hydrolyzed into retinol and deployed into circulation.¹⁵⁷ Once inside a cell, widely expressed alcohol dehydrogenases oxidize retinol into retinal, which can then bind to more selectively expressed retinaldehyde dehydrogenases (RALDHs) for oxidation into RA. Prior to its action, RA binds to nuclear receptors, including retinoic acid receptors (RARs) and retinoid X receptors.^158,159 Notably, all-trans RA exclusively binds retinoid X receptors via heterodimers with the RAR family, which consists of three receptors: RAR alpha (RAR&agr;), beta (RAR&bgr;), and gamma (RAR&ggr;).¹⁵⁸ Although RA is constitutively present in serum at low levels,¹⁶⁰ RALDH expression is tightly controlled.¹⁴⁷ GALT DCs express messenger ribonucleic acid for Aldh1a2, the gene encoding RALDH2.^119,161,162 In particular, basal Aldh1a2 expression in GALT DCs is enriched in CD103+ DC subsets.^162,163,164 Vitamin A itself is absolutely required for DC production of Aldh1a2 during homeostasis.^162,165,166 Although dispensable at steady state, microbial stimuli may affect RALDH expression during an inflammatory or infectious response.^167,168,169 In addition to DCs, several nonhematopoietic lineages within the GI tract and GALT, such as epithelia and stromal cells, share the capacity to synthesize RA (see Fig. 34.2).^{161,166,170,171,172}

Appropriate immune responses depend on the ability of effector and regulatory lymphocytes to home to the site of infection or injury. In this regard, DCs have been shown to foster lymphocyte migration into tissues where antigen was initially encountered.¹⁷³ One of the dominant actions of vitamin A on the mucosal immune system is associated with the capacity of its metabolite RA to promote the migration of effector cells to mucosal tissues. In particular, CD103+ DCs from the GI tract and GALT can induce the mucosal homing markers—integrin heterodimer &agr;4&bgr;7 and chemokine receptor CCR9—on stimulated effector T and B cells.^163,174,175 The insight that mucosal DCs triggered &agr;4&bgr;7 and CCR9 through their capacity to synthesize RA was based on the seminal observation that adding RA to T cells during activation selectively induced these gut homing markers.¹⁶¹ In a reciprocal fashion, blockade of RAR-mediated signaling and transcription in cultures containing GALT DCs reversed induction of &agr;4&bgr;7 and CCR9.^161,176 Recently, RA was revealed to predominantly affect the &agr;4 subunit of &agr;4&bgr;7 via binding of RAR&agr; to a retinoic acid receptor response element within the regulatory region of the &agr;4 gene.^177,178 An RA response element half-site was also recently discovered in the promoter region of CCR9, which RAR&agr;/retinoid X receptor heterodimers were able to bind to.¹⁷⁹ These data together with the prominent expression of Rar&agr;, the gene encoding RAR&agr;, in CD4+ T cells pinpoint RAR&agr; as a dominant mediator of CD4+ T-cell trafficking into mucosal sites.

Recent human data highlight the correlation between vitamin A status and T-cell function.¹⁸⁰ Impaired and/or dysregulated T-cell responses have been observed in various models of infection and vaccination strategies in the context of vitamin A and retinoid receptor deficiency.^{181,182,183,184,185} Experimentally, these results support a role for RA in the development of both Th1- and Th17-cell responses (Fig. 34.3).^{181,182,183,184,185} RA signaling appears to control the fate of T-cell immunity largely through RAR&agr; and RAR&ggr;.¹⁸⁴ In particular RA controls the capacity of CD4+ T cells to respond to antigen by regulating TCR signaling.¹⁸⁴ Further, RAR&agr; regulates the maturation of DCs in the GALT¹⁶⁵ and their ability to drive inflammatory responses in certain pathologic settings.¹⁸⁶ These data illustrate the ability of RA to regulate a network of innate and adaptive immune cell functions that, through nonredundant receptor signaling pathways, support functional immune responses. The discovery that RA served as a critical factor in the generation of IgA-secreting B cells offered further evidence of a multifactorial role for RA in mucosal immunity.⁶⁷ A number of studies have demonstrated the potent capacity of DCs from the intestinal LP, MLN, and Peyer patches to drive naïve B-cell differentiation into IgA+ B cells,^68,187,188 and the ability of stromal derived cells to support IgA+ class switching in activated B cells.^189,190 Synthesis of RA by GALT DCs was crucial for the generation of IgA by B cells, as antagonism of RA signaling significantly reduced IgA production.^68,188 Complementing this finding, addition of RA to DC cocultures in which DCs lacked the capacity to synthesize RA restored IgA+ production. Notably, microbial induced cytokines, such as IL-6, were also integral cofactors in this process.^68,188 This was also the case when stromal LP cells or stromal-derived follicular DCs from Peyer patches and MLN were assayed for their ability to foster the generation of IgA+ B cells.^189,190 In a manner analogous to peripheral DCs, peripheral follicular DCs were able to efficiently support IgA+ production only when treated with RA and in the context of Myd88-dependent microbial stimulus. 190 Intriguingly, this gain of function was dependent on the ability of RA signaling to induce secretion of TGF-&bgr; in peripheral follicular DCs.¹⁹¹ RA signaling was shown to promote a similar effect (ie, induction of TGF-&bgr; production) in
bone marrow-derived DCs via inhibition of suppressor of cytokine signaling 3 activity.¹⁹² These findings suggest interdependency between TGF-&bgr;- and RA-propagated signals in several cell lineages.

Another significant source of IgA production is B1 B cells, which contribute to homeostatic mucosal integrity and early responses to pathogens. RA was recently shown to be required for the steady-state maintenance of this compartment through direct regulation of NFATc1.⁸⁶ Combined with the capacity of RA to generate IgA+ B cells and facilitate their mucosal localization, vitamin A deficiency leads to severe decreases both in intestinal and serum IgA levels.^86,188 Altogether, these findings underscore the importance of RA in IgA responses and humoral immunity.

Resulting from its pleiotropic effects on the mucosal immune system, RA can also, in a context-dependent manner, contribute to mucosal inflammation (see Fig. 34.3). Almost 30 years ago, mice fed a diet high in vitamin A were observed to exhibit more vigorous responses against grafts and tumors.^193,194 These findings suggested that elevated retinoid levels could potentially predispose an individual to exuberant immune responses. In this regard, exposure to RA during inflammation contributed to loss of oral tolerance and reactivity to dietary glutens in a mouse model of celiac disease.^186,195 More specifically, RA was demonstrated to synergize with IL-15 to promote mucosal DC production of IL-12p70, which exacerbated responses to the glutenderived antigen, gliadin.¹⁸⁶ These data resonate with several reports, describing a possible association between pharmacologic retinoid treatment and spontaneous development of IBD, and point to vitamin A metabolic pathways as potential instigators of chronic inflammation.^196,197

The acquisition of oral tolerance—the active suppression of inflammatory responses against the myriad of antigens derived from food and the microbiota—is a fundamental aspect of the mucosal immune system. Multiple mechanisms are involved in the induction of oral tolerance.¹⁹⁸ Experimentally, oral administration of soluble proteins results in systemic tolerance that prevents the initiation of potentially damaging inflammatory responses upon rechallenge with the antigen in the periphery.^199,200,201 A complex regulatory network, including specialized populations of APCs, lymphocytes, and innate cytokines, controls GI tract homeostasis and converges to favor the induction of regulatory responses toward antigens present at mucosal sites. Early reports suggested that commensals played a role in this process as oral tolerance could not be induced in the absence of gut flora or gut flora-derived signals.^{198,202,203,204,205} Over the last few years, various populations of T_reg cells have been ascribed a central role in the induction of oral tolerance. CD4+ T_reg cells, in particular, play an essential role in maintaining peripheral immune tolerance as well as preventing autoimmunity and chronic inflammation with several subsets described on the basis of their origin, generation, and mechanism of action. In a simplistic manner, these cells can be divided into endogenous T_reg cells, represented by thymically derived Foxp3+ T_reg cells and inducible T_reg cells, such as IL-10-producing T_reg cells²⁰⁶ or inducible Foxp3+ T_reg (iT_reg) cells.^207,208 Although the precise mechanisms by which these cells coordinate their functions to maintain the delicate balance between immunity and tolerance remain incompletely understood, it can be conceived that collaboration and cross-talk among the aforementioned T_reg

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