Assay for 17 OHP:
Radioimmunoassay (RIA) .
Interpretation:
Caveats:
Baseline androgen levels return to baseline after 8 weeks of discontinuation of oral contraceptive pills (OCP) [4].
There is not enough data in regards to the effect of OCP on 17 OHP levels after ACTH stimulation.
Procedure:
Completed as outpatient.
1.
Obtain baseline blood sample for (17 OHP) and cortisol.
2.
Give cortrosyn 250 mcg IM.
3.

At 60 min, obtain sample for (17 OHP) and cortisol.

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